FastQCFastQC Report
Mon 16 Mar 2020
HKVH7DRXX_n01_dpr120.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHKVH7DRXX_n01_dpr120.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3790252
Sequences flagged as poor quality0
Sequence length20
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGG77200.20368038853353287No Hit
GGGTGTGTTGTGTTTTTGTG49740.1312313798660353No Hit

[WARN]Adapter Content

Can't analyse adapters as read length is too short

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGCCGTG401.745963E-414.0088991
CTCCTGG4150.014.0088991
GGCCGTG801.4915713E-1014.0088991
GCGCGGG401.745963E-414.0088991
TTCCTGT4300.014.0088991
TGCCTGG2150.014.0088991
CCCCGTT801.4915713E-1014.0088991
GCGCTTG801.4915713E-1014.0088991
GCTCGGG1250.014.0088991
GCGCTGT1250.014.0088991
CTTCGTT1550.014.0088991
CGGCTTG401.745963E-414.0088991
CCCCTTT1750.014.0088991
CCCCTTG1600.014.0088991
TCTCTTT3200.014.0088991
TCTCTTG1750.014.0088991
GCTCTTG3200.014.0088991
TCCCTTG1250.014.0088991
GCCCTTT1600.014.0088991
GCCCTGG2850.014.0088991
Produced by FastQC (version 0.11.5)