FastQCFastQC Report
Mon 16 Mar 2020
HKVH7DRXX_n01_dpr119.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHKVH7DRXX_n01_dpr119.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences21237
Sequences flagged as poor quality0
Sequence length20
%GC50

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGG300.14126289023873428No Hit
GTGTGTGTGTGTGTGTGTGT220.10359278617507181No Hit

[WARN]Adapter Content

Can't analyse adapters as read length is too short

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGTTGGC300.005685479414.0000013
TTTCTTT300.005685479414.0000016
TTTCTGT300.005685479414.0000016
TTTTTTC300.005685479414.0000013
TGCTGGT300.005685479414.0000017
GGCTGTG401.6293638E-414.0000017
TCTGTGG300.005685479414.0000018
TGGGCTG300.005685479414.0000015
TTGTGCT300.005685479414.0000014
TTTGCTG300.005685479414.0000015
TGTGCTT300.005685479414.0000015
TTTGTGC300.005685479414.0000013
GGCGGTG300.005685479414.0000017
TTGCTGG300.005685479414.0000016
GTGCTTT401.6293638E-414.0000016
GGGCTGT300.005685479414.0000016
TGGTCTG359.613022E-414.05
TTTTCTG452.7667693E-514.05
TTTTTCT452.7667693E-514.04
GCTGTGG359.613022E-414.08
Produced by FastQC (version 0.11.5)