FastQCFastQC Report
Mon 16 Mar 2020
HKVH7DRXX_n01_dpr118.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHKVH7DRXX_n01_dpr118.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13865
Sequences flagged as poor quality0
Sequence length20
%GC55

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGG460.3317706455102777No Hit
GTGTGTGTGTGTGTGTGTGT150.10818608005769925No Hit

[WARN]Adapter Content

Can't analyse adapters as read length is too short

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGTCTGG300.005553719614.06
TGGCTGG300.005553719614.06
TTGTCTG401.5651171E-413.9999995
GGGGGGT1550.0085989995.41935511
Produced by FastQC (version 0.11.5)