FastQCFastQC Report
Mon 16 Mar 2020
HKVH7DRXX_n01_dpr117.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHKVH7DRXX_n01_dpr117.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences32121
Sequences flagged as poor quality0
Sequence length20
%GC56

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGG420.13075558046138042No Hit

[WARN]Adapter Content

Can't analyse adapters as read length is too short

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTGTCTG300.005732496314.0140415
GTTTGGC300.005732496314.0140413
TTTTCTG300.005732496314.0140415
TTGGCTG359.731503E-414.0140415
TGTGGGC300.005732496314.0140413
GGTGGTC359.731503E-414.0140413
TTGGTCT300.005732496314.0140414
GGTGGGC300.005732496314.0140413
GGGTGGC359.731503E-414.0140413
GTGTGCG300.005732496314.0140414
GGTGTGC359.731503E-414.0140413
GTGGGCT452.8295002E-514.014044
TGGGCTG452.8295002E-514.014045
TTTGGCT401.6572622E-414.014044
TTTGCTG452.8295002E-514.014045
TGTGCTG452.8295002E-514.014045
GGCGGGG359.847512E-413.9922127
TGGCTGT300.005792322613.9922126
GCTGGGG401.6794851E-413.9922118
TTGCTGT401.6794851E-413.9922116
Produced by FastQC (version 0.11.5)