FastQCFastQC Report
Mon 16 Mar 2020
HKVH7DRXX_n01_dpr116.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHKVH7DRXX_n01_dpr116.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1060429
Sequences flagged as poor quality0
Sequence length20
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGG11730.11061560934301118No Hit

[WARN]Adapter Content

Can't analyse adapters as read length is too short

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCCTGT652.7655915E-814.0107611
TTCCTGG1300.014.0107611
GCTCGTG652.7655915E-814.0107611
CTTCGGG652.7655915E-814.0107611
CTGCTGG652.7655915E-814.0107611
TCCCTGT652.7655915E-814.0107611
CTCCTTT453.0107783E-514.010761
CTCCTTG453.0107783E-514.010761
CGCCTTT300.00590468814.010761
TTCCGTT350.001011959614.010761
CTCCTGG801.4915713E-1014.010761
CGCCTGG601.5828118E-714.010761
TTCCGGT453.0107783E-514.010761
TTCCGGG300.00590468814.010761
GTCCGTT401.7421252E-414.010761
CCTCGTG559.0788853E-714.010761
CCGCTTT453.0107783E-514.010761
CCGCTTG300.00590468814.010761
GTCCGGT601.5828118E-714.010761
CCTCGGT300.00590468814.010761
Produced by FastQC (version 0.11.5)