FastQCFastQC Report
Mon 16 Mar 2020
HKVH7DRXX_n01_dpr115.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHKVH7DRXX_n01_dpr115.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1136871
Sequences flagged as poor quality0
Sequence length20
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGG14040.1234968611214465No Hit

[WARN]Adapter Content

Can't analyse adapters as read length is too short

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCCTTT505.23143E-614.0081871
CGCCTGT505.23143E-614.0081871
CGCCTGG505.23143E-614.0081871
CCTCGTG505.23143E-614.0081871
CCGCTTT652.7728674E-814.0081871
GTCCGGG652.7728674E-814.0081871
CCGCTGT559.0993854E-714.0081871
TTCCTTT1000.014.0081871
TTCCTGG1000.014.0081871
TGCCTGT758.5128704E-1014.0081871
TGCCTGG505.23143E-614.0081871
CCTCTTT1100.014.0081871
GGCCTTT559.0993854E-714.0081871
GTCCTGT1100.014.0081871
CCTCTGT505.23143E-614.0081871
CTTCGTT652.7728674E-814.0081871
CTGCTGT758.5128704E-1014.0081871
CTGCTGG758.5128704E-1014.0081871
TCTCTTG505.23143E-614.0081871
TCTCTGT559.0993854E-714.0081871
Produced by FastQC (version 0.11.5)