FastQCFastQC Report
Mon 16 Mar 2020
HKVH7DRXX_n01_dpr114.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHKVH7DRXX_n01_dpr114.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences88459
Sequences flagged as poor quality0
Sequence length20
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGG2040.2306153133089906No Hit

[WARN]Adapter Content

Can't analyse adapters as read length is too short

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGTTCGG758.094503E-1014.0050925
TTTTGTC401.7197241E-414.0050913
TGTTGTC452.9611047E-514.0050913
TTGTCTT300.005864066514.0050915
TTGTCTG558.8514025E-714.0050915
TGGTCTG1100.014.0050915
TCCTTTG300.005864066514.0050912
TGTTGGC505.113323E-614.0050913
TTTTGCT801.4188117E-1014.0050914
TTTTGCG601.5353726E-714.0050914
TGTTGCT452.9611047E-514.0050914
TTGTCGT452.9611047E-514.0050915
TTGTTCT452.9611047E-514.0050914
TTGTTCG452.9611047E-514.0050914
TGGTTCT452.9611047E-514.0050914
GGGTCTG558.8514025E-714.0050915
GTTTGGC452.9611047E-514.0050913
GTTTGCT652.6679118E-814.0050914
GTGTCGT300.005864066514.0050915
GTGTCGG401.7197241E-414.0050915
Produced by FastQC (version 0.11.5)