FastQCFastQC Report
Mon 16 Mar 2020
HKVH7DRXX_n01_dpr113.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHKVH7DRXX_n01_dpr113.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8047296
Sequences flagged as poor quality0
Sequence length20
%GC57

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGG149050.18521749417444072No Hit
GTGTGTGTGTGTGTGTGTGT120170.1493296630321539No Hit

[WARN]Adapter Content

Can't analyse adapters as read length is too short

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCCTTT5050.014.0091721
CCTCTGT11700.014.0091721
CTGCTTT4450.014.0091721
CCCCTTT3250.014.0091721
TCCCGGT2950.014.0091721
GCCCGTG5900.014.0091721
CTCCGGG4750.014.0091721
GCCCTTG7700.014.0091721
CTCCTGG15550.014.00917151
CGCCTGT1300.014.00917151
CGCCTGG2750.014.00917151
CCTCGTT3750.014.00917151
GTCCGTG9250.014.00917151
CCTCGTG6550.014.00917151
GGCCGTG5300.014.00917151
CCGCTTG1150.014.00917151
CCTCGGT3950.014.00917151
CCTCGGG4850.014.00917151
GGCCGGT2650.014.00917151
CCGCTGT1300.014.00917151
Produced by FastQC (version 0.11.5)