FastQCFastQC Report
Mon 16 Mar 2020
HKVH7DRXX_n01_dpr112.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHKVH7DRXX_n01_dpr112.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4650616
Sequences flagged as poor quality0
Sequence length20
%GC57

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGG115600.24856922179771454No Hit

[WARN]Adapter Content

Can't analyse adapters as read length is too short

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCCTTG852.5465852E-1114.0102441
TTCCGTT950.014.0102441
TTCCGTG1700.014.0102441
GTCCGTG4550.014.0102441
GGCCGTT852.5465852E-1114.0102441
GTCCGGG3550.014.0102441
GGCCGGG3500.014.0102441
TTCCTTG3700.014.0102441
TGCCTTT1700.014.0102441
TGCCTTG2500.014.0102441
TTCCTGT4850.014.0102441
CCTCTGG8350.014.0102441
TCTCGGT1250.014.0102441
TCGCTGG852.5465852E-1114.0102441
CTGCTTT1900.014.0102441
CCCCTTT2350.014.0102441
CGGCTGG852.5465852E-1114.0102441
CCCCTGG6800.014.0102441
GCCCGTG3450.014.0102441
GCTCTGG9700.014.0102441
Produced by FastQC (version 0.11.5)