FastQCFastQC Report
Mon 16 Mar 2020
HKVH7DRXX_n01_dpr111.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHKVH7DRXX_n01_dpr111.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences351449
Sequences flagged as poor quality0
Sequence length20
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGG6750.19206200615167493No Hit
GTGTGTGTGTGTGTGTGTGT4760.13543928137510705No Hit

[WARN]Adapter Content

Can't analyse adapters as read length is too short

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCCTGT401.7384854E-414.0092551
CTCCTGG350.001010414614.0092551
TTCCTTT652.7486749E-814.0092551
TTCCTTG401.7384854E-414.0092551
GTCCTTT350.001010414614.0092551
GTCCTGG453.0025469E-514.0092551
CTTCGTT401.7384854E-414.0092551
CTGCTGG350.001010414614.0092551
TCTCTGT401.7384854E-414.0092551
CTTCTTG704.807589E-914.0092551
TCCCTGT401.7384854E-414.0092551
TCCCTGG453.0025469E-514.0092551
GCCCTTG350.001010414614.0092551
GCCCTGG801.4733814E-1014.0092551
TTCCTGT601.5746446E-714.0092541
TTCCTGG300.005898512414.0092541
GTCCTTG300.005898512414.0092541
CCTCTTG505.2026317E-614.0092541
GTCCTGT505.2026317E-614.0092541
CCTCTGT559.040141E-714.0092541
Produced by FastQC (version 0.11.5)