FastQCFastQC Report
Mon 16 Mar 2020
HKVH7DRXX_n01_dpr110.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHKVH7DRXX_n01_dpr110.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1136496
Sequences flagged as poor quality0
Sequence length20
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGG16930.14896664836479848No Hit

[WARN]Adapter Content

Can't analyse adapters as read length is too short

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCCTGT1050.014.0137431
CTCCTGG1050.014.0137431
GTCCGTT505.209993E-614.0137431
GTCCGTG350.001010410814.0137431
CCTCGTG300.00589670514.0137431
GGCCGTG300.00589670514.0137431
CCTCGGG350.001010410814.0137431
TCGCGTT350.001010410814.0137431
CCGCTGT350.001010410814.0137431
CCGCTGG300.00589670514.0137431
TTCCTTT1000.014.0137431
TTCCTTG758.458301E-1014.0137431
TGCCTTT601.5790465E-714.0137431
TGCCTGG758.458301E-1014.0137431
GTCCTTT505.209993E-614.0137431
GTCCTTG505.209993E-614.0137431
GGCCTTT300.00589670514.0137431
GGCCTTG300.00589670514.0137431
CTGCGGG704.827598E-914.0137431
TCTCGTT300.00589670514.0137431
Produced by FastQC (version 0.11.5)