FastQCFastQC Report
Mon 16 Mar 2020
HKVH7DRXX_n01_dpr108.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHKVH7DRXX_n01_dpr108.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2819770
Sequences flagged as poor quality0
Sequence length20
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGTGTGTGTGTGTGTGTGT68830.24409792288023494No Hit
GGGTTTGGGTTTGGGTTTGG49260.17469509924568316No Hit
GGGGGGGGGGGGGGGGGGGG36000.12766998726846515No Hit

[WARN]Adapter Content

Can't analyse adapters as read length is too short

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTCCGTG852.7284841E-1114.0077911
CTCCTTT1750.014.007791
CGCCTTG350.001014316514.007791
CTCCTGT3900.014.007791
TGCCGTG350.001014316514.007791
CGCCTGT704.8694346E-914.007791
CGCCTGG559.1160837E-714.007791
GTCCGTT1100.014.007791
GGCCGTT505.239579E-614.007791
GGCCGTG300.00591620914.007791
CCGCTTT300.00591620914.007791
GTCCGGT1000.014.007791
CCTCGGT505.239579E-614.007791
GTCCGGG1100.014.007791
GGCCGGG559.1160837E-714.007791
CCGCTGG300.00591620914.007791
TCGCGGG350.001014316514.007791
GCGCGTG350.001014316514.007791
TTCCTTG2000.014.007791
TGCCTTG758.54925E-1014.007791
Produced by FastQC (version 0.11.5)