FastQCFastQC Report
Mon 16 Mar 2020
HKVH7DRXX_n01_dpr107.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHKVH7DRXX_n01_dpr107.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11190318
Sequences flagged as poor quality0
Sequence length20
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGG214180.19139759924606253No Hit
GTGTGTGTGTGTGTGTGTGT210720.1883056406439924No Hit

[WARN]Adapter Content

Can't analyse adapters as read length is too short

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCCTGG24950.014.0087071
CCCCTTG10400.014.0087071
GCTCTGT20800.014.0087071
GTCATTG300.005915093214.0087061
CTCCTTG13050.014.0087061
TTCCGGT2150.014.0087061
CCTCTTT6450.014.0087061
CCCCGTT2650.014.0087061
TCTCGTG5100.014.0087061
CCTCTGG20200.014.0087061
CTTATGT300.005915093214.0087061
CTTCGTT4300.014.0087061
CTTCGTG7700.014.0087061
CTTCGGG2750.014.0087061
CCCCTGG18200.014.0087061
TCCCGGT2500.014.0087061
TCCCGGG3700.014.0087061
CGTCTTT905.456968E-1214.0087061
CGTCTTG2300.014.0087061
CTCCGTT3650.014.0087061
Produced by FastQC (version 0.11.5)