FastQCFastQC Report
Mon 16 Mar 2020
HKVH7DRXX_n01_dpr106.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHKVH7DRXX_n01_dpr106.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8971114
Sequences flagged as poor quality0
Sequence length20
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGTGTGTGTGTGTGTGTGT198720.2215109517056633No Hit
GGGGGGGGGGGGGGGGGGGG151520.16889764192050172No Hit
GGGTTTGGGTTTGGGTTTGG117710.13121001472057986No Hit

[WARN]Adapter Content

Can't analyse adapters as read length is too short

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCCTTT950.014.00785351
TTCCGTT1450.014.00785351
TTCCGTG2300.014.00785351
CGCCTGG2900.014.00785351
GGCCGTT950.014.00785351
GTCCGGT3800.014.00785351
CCTCGGT2750.014.00785351
TTCCTGG11250.014.00785351
GGCCTTG6950.014.00785351
TCTCGTT2450.014.00785351
TCTCGGT2150.014.00785351
GCGCTTG2000.014.00785351
CCCCTTT3300.014.00785351
CTGCTGT8350.014.00785351
TCCCGGG2700.014.00785351
CTCCGTT2300.014.00785351
CTCCGTG5750.014.00785351
TCCCTGT11150.014.00785351
CTCCTTT5700.014.0078531
CGCCTTG1050.014.0078531
Produced by FastQC (version 0.11.5)