FastQCFastQC Report
Mon 16 Mar 2020
HKVH7DRXX_n01_dpr105.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHKVH7DRXX_n01_dpr105.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12583056
Sequences flagged as poor quality0
Sequence length20
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGTGTGTGTGTGTGTGTGT233460.1855352149748042No Hit
GGGGGGGGGGGGGGGGGGGG232080.18443850206182028No Hit

[WARN]Adapter Content

Can't analyse adapters as read length is too short

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTGAGTG300.00591368114.0092311
CGCCTTT1000.014.0092311
CGCCTTG2400.014.0092311
GTCCGTG12600.014.0092311
CCGCTTT559.112737E-714.0092311
CCTCGGT6450.014.0092311
TTCCTTG8800.014.0092311
TTCCTGG14200.014.0092311
CCTCTTT9600.014.0092311
CCTCTTG13600.014.0092311
GTCCTGG25900.014.0092311
CCCCGGT3550.014.0092311
TCGCTTT559.112737E-714.0092311
CGTCGTT401.7461203E-414.0092311
CGGCTTT505.237467E-614.0092311
CCCCTTT6400.014.0092311
TCCCGTT2000.014.0092311
CGTCTTG2200.014.0092311
CTCCGTG10350.014.0092311
CGCCGTT801.5097612E-1014.0092311
Produced by FastQC (version 0.11.5)