FastQCFastQC Report
Mon 16 Mar 2020
HKVH7DRXX_n01_dpr103.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHKVH7DRXX_n01_dpr103.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3344253
Sequences flagged as poor quality0
Sequence length20
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGTGTGTGTGTGTGTGTGT42990.12854888670205275No Hit
GGGGGGGGGGGGGGGGGGGG40850.12214984930864979No Hit

[WARN]Adapter Content

Can't analyse adapters as read length is too short

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGCCTGG3100.014.0102821
TCCCTTT1550.014.0102821
CTCCTTT1750.014.0102811
CGCCTTT801.4915713E-1014.0102811
TTCCGTT1200.014.0102811
CTCCTGG3150.014.0102811
CGCCTGT1300.014.0102811
CCGCTTG758.5310603E-1014.0102811
CCTCGGT801.4915713E-1014.0102811
CCTCGGG652.7739588E-814.0102811
TCGCGTT401.7444049E-414.0102811
GCGCGGG300.00590954414.0102811
TTCCTGT4500.014.0102811
CTGCGTT350.001013023714.0102811
GTCCTTT3750.014.0102811
CCTCTTG1750.014.0102811
GGCCTTT1150.014.0102811
CTGCGGT401.7444049E-414.0102811
TCTCGTT704.8585207E-914.0102811
TCGCTTT652.7739588E-814.0102811
Produced by FastQC (version 0.11.5)