FastQCFastQC Report
Mon 16 Mar 2020
HKVH7DRXX_n01_dpr102.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHKVH7DRXX_n01_dpr102.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2184178
Sequences flagged as poor quality0
Sequence length20
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGG26080.11940418775392848No Hit
GTGTGTGTGTGTGTGTGTGT23510.10763774747296238No Hit

[WARN]Adapter Content

Can't analyse adapters as read length is too short

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCCTGT758.54925E-1014.0071481
CGCCTGG601.5904334E-714.0071481
TTCCGGT601.5904334E-714.0071481
TTCCGGG852.7284841E-1114.0071481
GTCCGTG758.54925E-1014.0071481
GGCCGTG758.54925E-1014.0071481
CCGCTTG300.00591744314.0071481
CCTCGGT300.00591744314.0071481
CCTCGGG601.5904334E-714.0071481
CCGCTGG758.54925E-1014.0071481
TCGCGGT300.00591744314.0071481
GCGCGTT300.00591744314.0071481
GCGCGTG300.00591744314.0071481
GCGCGGT300.00591744314.0071481
GCGCGGG300.00591744314.0071481
TGCCTTT601.5904334E-714.0071481
CTGCGTT300.00591744314.0071481
CCTCTGG1200.014.0071481
TCGCTTT601.5904334E-714.0071481
TCTCGGG601.5904334E-714.0071481
Produced by FastQC (version 0.11.5)