FastQCFastQC Report
Mon 16 Mar 2020
HKVH7DRXX_n01_dpr101.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHKVH7DRXX_n01_dpr101.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13916182
Sequences flagged as poor quality0
Sequence length20
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGTGTGTGTGTGTGTGTGT301560.2166973671370495No Hit
GGGGGGGGGGGGGGGGGGGG249610.17936672572980145No Hit

[WARN]Adapter Content

Can't analyse adapters as read length is too short

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTCTGG3500.014.0084541
CGCCTTG2350.014.0084531
CTCCTGG27500.014.0084531
CGCCTGT2100.014.0084531
CCTCGTT4800.014.0084531
GTCCGTG10800.014.0084531
CCGCTTG1050.014.0084531
CCGCTGT1700.014.0084531
TCGCGGT505.2405685E-614.0084531
TCGCGGG300.00591589614.0084531
CGGCGTG905.456968E-1214.0084531
CCCCGTG5400.014.0084531
CGGCGGT453.0206307E-514.0084531
CCTCTGT21200.014.0084531
CCCCTTT7150.014.0084531
CTTCGGG4150.014.0084531
TCTCTGG18800.014.0084531
GCCCGTG8250.014.0084531
GCCCGGT4700.014.0084531
CTCATGT300.00591589614.0084531
Produced by FastQC (version 0.11.5)