FastQCFastQC Report
Mon 16 Mar 2020
HKVH7DRXX_n01_dpr100.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHKVH7DRXX_n01_dpr100.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7730570
Sequences flagged as poor quality0
Sequence length20
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGG131430.170013336662109No Hit
GTGTGTGTGTGTGTGTGTGT116370.1505322375969689No Hit

[WARN]Adapter Content

Can't analyse adapters as read length is too short

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGCCTGT7500.014.0077681
CTCCTTT5450.014.0077671
TTCCGTG2500.014.0077671
CTCCTGT12550.014.0077671
TTCCGGG1500.014.0077671
TGCCGGG1250.014.0077671
CCTCGTT2600.014.0077671
CCTCGTG6000.014.0077671
CCGCTTG704.8748916E-914.0077671
GTCCGGT4100.014.0077671
CCTCGGT4050.014.0077671
CCTCGGG4250.014.0077671
GCGCGTT652.7823262E-814.0077671
TTCCTTG6250.014.0077671
TTCCTGG9850.014.0077671
CTGCGTG3300.014.0077671
CCTCTTT4050.014.0077671
GTCCTTG11600.014.0077671
CCTCTTG8450.014.0077671
CCCCGTT1600.014.0077671
Produced by FastQC (version 0.11.5)