FastQCFastQC Report
Tue 5 Jan 2021
HKVGTAFX2_n02_vk11-9.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHKVGTAFX2_n02_vk11-9.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5127911
Sequences flagged as poor quality0
Sequence length76
%GC38

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG215430.42011259555791824No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAAGGGG277750.046.302970
CGAGATC52100.035.2353135
TCTCGGG39100.034.63424343
CTAGATC38650.034.625695
CCGGATC85050.034.3909455
CGGATCA86400.033.97639556
TGTCGTG21100.033.949621
CTCGGGG42700.033.43355644
GCGAGAT19000.032.286074
CATTAAA367150.031.77093161
CAAGATC71150.031.7099515
CGCGTAG5100.031.6530442
CGCATAG3350.031.4270972
CCGTATC286100.031.34316655
CGTATCA286550.031.33179956
CGCCGGA116700.031.3288853
TCATTAA366200.031.18456660
GGATCAT77600.031.02157
CGCGAAA6050.030.7615151
GGGTCGC61200.030.64438649
Produced by FastQC (version 0.11.5)