FastQCFastQC Report
Tue 5 Jan 2021
HKVGTAFX2_n02_vk11-8.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHKVGTAFX2_n02_vk11-8.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5516403
Sequences flagged as poor quality0
Sequence length76
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG226610.41079304757103496No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAAGGGG75900.029.6878370
CCGGATC18300.025.6198355
CCGTATC58500.025.5384555
CGCCGTA63300.025.26009653
GTCGCCG89750.024.79369451
CGCCGGA23250.024.52941353
CGCGAAA10450.024.5227431
CGGATCA19200.024.23754756
TCGCCGT66200.024.2063952
CGTATCA61750.024.13853656
TCGCCGG27100.023.75587552
CTCGGTG85350.023.24584844
CGGCAAG16000.023.0373081
GGTCGCC106000.023.00748450
GATCTCG91850.022.82071541
ATCTCGG93300.022.72859442
GCCGTAT71550.022.59221554
TCTCGGT87300.022.44664443
TGGTCGC99800.022.19357149
AGATCTC98300.021.46610640
Produced by FastQC (version 0.11.5)