FastQCFastQC Report
Tue 5 Jan 2021
HKVGTAFX2_n02_vk11-7.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHKVGTAFX2_n02_vk11-7.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5013117
Sequences flagged as poor quality0
Sequence length76
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG242200.4831325500681512No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAAGGGG147100.040.17425570
CGCGAAA5900.032.1331251
CGAGATC25400.029.7918365
CCGGATC46850.029.05078355
CGGATCA47800.028.69414756
CGTATCA149250.027.5460156
CCGTATC149850.027.38792255
CTAGATC25150.027.3020535
CATTAAA198850.027.08023861
CGCGGCC1455.984475E-1026.5425466
TCATTAA200450.026.30576960
CGCCGTA166600.026.10415353
CGCCAAG7300.025.970611
CGCCGGA62600.025.82147853
TGTCGTG16600.025.8025721
ATCTCGG234850.025.73291242
GATCTCG230900.025.64294841
GTATCAT153900.025.28166457
AGATCTC234450.025.25466540
CTCGGTG221650.025.24380944
Produced by FastQC (version 0.11.5)