FastQCFastQC Report
Tue 5 Jan 2021
HKVGTAFX2_n02_vk11-6.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHKVGTAFX2_n02_vk11-6.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5548289
Sequences flagged as poor quality0
Sequence length76
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG286290.515996913643107No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGGATC26400.028.36131355
AAAGGGG101900.027.77735170
CGGATCA27700.027.15781256
CCGTATC65850.026.30061555
CGCCGTA66550.026.23426853
CGCCGGA29500.025.85538153
CGTATCA68500.025.33536556
GTCGCCG109100.025.27051251
ATCTCGG109350.025.0559242
GATCTCG109600.024.83913241
GGTCGCC115400.024.83214250
TCGCCGT72850.024.63793652
TCGCCGG39400.024.50921252
GCCGTAT74700.024.26216554
GCCGGAT36000.024.00572654
TGGTCGC102200.023.82858849
CTCGGTG102450.023.66926244
AGATCTC113600.023.47167440
TCTCGGT103050.023.32771543
AAGGGGG64400.022.9267870
Produced by FastQC (version 0.11.5)