FastQCFastQC Report
Tue 5 Jan 2021
HKVGTAFX2_n02_vk11-5.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHKVGTAFX2_n02_vk11-5.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5359596
Sequences flagged as poor quality0
Sequence length76
%GC38

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG252790.47165868472175887No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAAGGGG191500.041.5471470
CCGGATC56200.032.18679455
CGGATCA57750.031.38466356
CGAGATC31600.029.3765965
CGCCGGA77750.028.35121753
CGGCAAG10250.027.4080141
CGTATCA179400.027.28569256
CCGTATC180300.027.03204355
TCGCCGG89750.026.82190552
CAAGATC48200.026.8177285
TGTCGTG20450.026.6164321
CATTAAA249800.026.54339661
GCCGGAT87050.026.407854
CGCGCAG2800.026.3373891
CTAGATC28950.026.2575285
CGCCGTA200950.026.1002853
GGATCAT57950.026.08374857
ATCTCGG277350.026.02814342
GATCTCG272950.025.97265241
GTCGCCG295600.025.88665451
Produced by FastQC (version 0.11.5)