FastQCFastQC Report
Tue 5 Jan 2021
HKVGTAFX2_n02_vk11-4.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHKVGTAFX2_n02_vk11-4.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5452722
Sequences flagged as poor quality0
Sequence length76
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG243410.4464008984870309No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAAGGGG209050.041.7086970
CCGGATC62000.033.80428755
CGGATCA64250.032.7853656
CTCGGGG28450.032.47059244
TCTCGGG26750.032.1810243
CGCCGGA83500.031.8463653
CGAGATC38950.031.4739825
CGCGCAG2600.031.0638891
TCGCCGG95700.030.30953252
CATTAAA278600.029.8039161
TCATTAA274750.029.40646660
GGATCAT58250.029.37443457
GCCGGAT94300.029.27560854
CCGTATC219500.028.9479655
CGTATCA220500.028.9449556
GGGTCGC40350.028.7914249
ATCATTA279250.028.18076959
GTATCAT211950.028.1314957
ATCTCGG325000.027.95231242
GATCTCG321150.027.9382141
Produced by FastQC (version 0.11.5)