FastQCFastQC Report
Tue 5 Jan 2021
HKVGTAFX2_n02_vk11-3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHKVGTAFX2_n02_vk11-3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4156594
Sequences flagged as poor quality0
Sequence length76
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG195700.47081817468821824No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAAGGGG232200.036.7804670
TGTCGTG16700.032.5886421
CGCGTAG3350.032.469012
CGCGAAA4900.030.8122481
CTAGATC28400.030.2173335
CCGGATC73750.029.84009755
CGGATCA74100.029.79537856
CGAGATC31950.029.6006555
AAGGGGG127000.029.53266570
CATTAAA240500.029.46065561
TCATTAA237600.029.1428460
CGTATCA169950.028.84394656
CCGTATC170400.028.78657555
CGCGCAG2450.028.6625561
GGATCAT67200.028.48103557
GTGCGTC3350.028.2989271
CGCCGGA85550.027.9732253
CAAGATC50300.027.9244615
CGGCAAG8600.027.7626861
CGCCGTA180850.027.49045653
Produced by FastQC (version 0.11.5)