FastQCFastQC Report
Tue 5 Jan 2021
HKVGTAFX2_n02_vk11-2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHKVGTAFX2_n02_vk11-2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5136164
Sequences flagged as poor quality0
Sequence length76
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG227960.4438331797816425No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAAGGGG129050.032.7493570
CCGGATC32950.031.11133455
CGGATCA34500.030.01898256
CGCCGGA39850.027.65595853
CGCCGTA87950.026.8120853
CCGTATC83650.026.60097755
GCCGGAT45400.026.27876554
CTCGGGG25400.026.17442144
CGTATCA86600.026.09984856
TCGCCGG52550.025.69928652
GTCGCCG144000.025.68141651
TCTCGGG24000.025.66061643
CGCGCAG2200.025.5380921
ATCTCGG143550.025.40004242
GATCTCG141600.025.37914841
AAGGGGG73800.025.17282370
GCCGTAT96900.025.05775554
AGATCTC141550.024.96810540
GGGTCGC27000.024.8808449
TCGCCGT94400.024.8318652
Produced by FastQC (version 0.11.5)