FastQCFastQC Report
Tue 5 Jan 2021
HKVGTAFX2_n02_vk11-20.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHKVGTAFX2_n02_vk11-20.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4237930
Sequences flagged as poor quality0
Sequence length76
%GC36

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG160600.37895859535197607No Hit
TGGGGTGGTATAGTCCGCATTGGGATGTTACTTTCCTGTTATGGCATGGA44860.10585356530192806No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGGATC50050.041.87375355
CGGATCA51200.040.66228556
TCTCGGG33050.040.55303243
CTCGGGG34650.040.4978644
AAAGGGG219650.039.77512770
GCCGGAT60800.039.30395554
CGCCGGA55950.038.95893553
GATCGCG19850.038.4323741
ATCGCGG20000.038.319142
GGGTCGC38750.038.28771249
GTCGCCG215100.037.90004751
CGCCGTA141000.037.7671953
ATCTCGG210500.037.7542842
GATCTCG208300.037.71622541
CCGTATC137800.037.65399655
AGATCTC205700.037.5634940
TCGCCGT145450.037.5498652
TCGCCGG71250.037.51695652
CGTATCA139600.037.14561556
AAGGGGG98850.037.05904870