FastQCFastQC Report
Tue 5 Jan 2021
HKVGTAFX2_n02_vk11-1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHKVGTAFX2_n02_vk11-1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4638636
Sequences flagged as poor quality0
Sequence length76
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG232330.5008584420075212No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAAGGGG154150.043.5127770
CCGGATC39450.034.14731655
CGGATCA40800.032.93203756
CGCGCAG2250.032.7706831
CGCCGGA55400.030.56913453
CCGTATC143150.030.4802155
CGTATCA145300.030.1980956
TCGCCGG65750.030.06737752
TCTCGGG20100.029.94355443
CGCCGTA160750.029.18941153
GTCGCCG231800.028.92177451
CATTAAA196950.028.8521161
GGGTCGC29000.028.71678749
GCCGGAT62200.028.63291454
ATCTCGG221900.028.41666642
GATCTCG219150.028.3903441
CTCGGGG23050.028.38875244
CGAGATC27550.028.3499625
TCATTAA197050.028.25148460
TCGCCGT166500.028.20208252
Produced by FastQC (version 0.11.5)