FastQCFastQC Report
Tue 5 Jan 2021
HKVGTAFX2_n02_vk11-19.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHKVGTAFX2_n02_vk11-19.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4739760
Sequences flagged as poor quality0
Sequence length76
%GC34

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG152670.3221049167046433No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAAGGGG192400.046.8432370
CTCGGGG23750.043.46343644
TCTCGGG22200.042.55843443
CCGGATC48650.041.28174655
TCGCCGG69100.040.70969452
GGGTCGC30200.040.55121649
CGGATCA50800.039.67275256
CGCCGGA62500.039.4105553
GCCGGAT65100.038.91185854
GTCGCCG229550.038.0284451
GATCTCG217950.037.4244741
ATCTCGG223300.037.28118542
CGGGGGT41200.037.19996646
AGATCTC221650.036.9110340
CCGTATC150450.036.69811655
CTCGGTG205150.036.45009644
CGCCGTA160850.036.43456353
TCGCCGT162350.036.2487952
TCTCGGT204750.036.19735743
CGTATCA153400.036.1752456
Produced by FastQC (version 0.11.5)