FastQCFastQC Report
Tue 5 Jan 2021
HKVGTAFX2_n02_vk11-18.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHKVGTAFX2_n02_vk11-18.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3432635
Sequences flagged as poor quality0
Sequence length76
%GC38

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG120310.3504887644622862No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAAGGGG387000.050.79009670
CTCGGGG43000.043.941844
TCTCGGG40950.043.6655143
CCGGATC96000.042.4225155
CGCGTAG2400.042.3996932
CGGATCA96650.042.10284456
AAGGGGG108000.041.79017670
TCGCCGG133450.041.3722752
CGCCGGA121050.041.0435153
GCCGGAT126850.041.01487754
GGGTCGC56600.040.80127749
CCCGGTC7550.040.78088454
CGCGCAG1550.040.7769931
CGGGGGT68950.039.3296446
GGATCAT85850.039.08572857
CCGGTCA8150.038.20735555
GATCGCG27500.038.047241
ATCGCGG28300.037.59046642
GCAGATC49900.037.5824474
GGGGTCG61850.037.45218748