FastQCFastQC Report
Tue 5 Jan 2021
HKVGTAFX2_n02_vk11-17.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHKVGTAFX2_n02_vk11-17.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3813748
Sequences flagged as poor quality0
Sequence length76
%GC35

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG130410.34194708197814855No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCGCAG1950.043.2121771
AAAGGGG281800.041.99094470
TCTCGGG38650.040.64992543
CTCGGGG42100.040.64176644
AAGGGGG115050.040.1734670
CCGGATC71600.040.16761855
CGGATCA72150.039.86194256
GGGTCGC46000.039.6280749
TCGCCGG100650.038.13330552
CGCCGGA83300.038.0955253
GCCGGAT87900.037.77414754
CCCGGTC4700.037.2211254
GATCGCG25200.037.2151641
GTCGCCG291500.037.1238551
GATCTCG280500.036.92704441
ATCGCGG26250.036.9268142
ATCTCGG283650.036.83819642
AGATCTC278050.036.61057340
CGCCGTA187600.036.59129353
CGGGGGT62600.036.44341746
Produced by FastQC (version 0.11.5)