FastQCFastQC Report
Tue 5 Jan 2021
HKVGTAFX2_n02_vk11-16.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHKVGTAFX2_n02_vk11-16.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5946776
Sequences flagged as poor quality0
Sequence length76
%GC37

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG195380.32854777109479155No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCGCCG62350.035.18528751
CGCCGTA43900.033.555153
TCGCCGT44700.033.11111552
TCGCCGG22000.032.92218452
GGTCGCC73100.032.35824250
ATCTCGG64450.032.1962742
CTCGGTG59250.032.0093944
CCGTATC43150.031.86811355
GATCTCG64400.031.67817941
CGCCGGA20000.031.66573153
TGGTCGC67400.030.8377149
AAAGGGG79500.030.6760470
CGTATCA46650.029.62741356
GCCGTAT51300.029.39710254
CCGGATC17550.029.30790955
GTGGTCG72500.028.86221948
AGATCTC71700.028.64838240
GGTGGTC76000.027.3486447
CGGATCA19650.026.88825856
GCCGGAT24650.026.82807554
Produced by FastQC (version 0.11.5)