FastQCFastQC Report
Tue 5 Jan 2021
HKVGTAFX2_n02_vk11-15.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHKVGTAFX2_n02_vk11-15.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5572524
Sequences flagged as poor quality0
Sequence length76
%GC37

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG190600.3420353146976128No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCGCCG43850.031.11876551
GGTCGCC49050.030.31827750
TCGCCGG15500.030.022852
ATCTCGG46250.029.58252542
GATCTCG47000.028.8126641
CTCGGTG41900.028.72887244
CGCCGTA31550.028.7231253
CTCGGGG8250.028.41809744
TCGCCGT32600.028.11997652
TGGTCGC46700.027.87280749
CGCCGGA14150.026.9526453
AAAGGGG64650.025.92284270
CCGTATC31600.025.79906555
GTGGTCG50500.025.7066248
AGATCTC53000.025.35300640
GTCGCCC9650.025.01937350
TCTCGGG9000.024.88328643
CCGGATC13500.024.10373355
CGTGTAG70350.023.93441422
TCGTGTA74750.023.36851721
Produced by FastQC (version 0.11.5)