FastQCFastQC Report
Tue 5 Jan 2021
HKVGTAFX2_n02_vk11-14.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHKVGTAFX2_n02_vk11-14.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4150577
Sequences flagged as poor quality0
Sequence length76
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG193640.4665375440571275No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAAGGGG193200.045.0195570
CGCGGCC1055.5479177E-1033.32058366
CCGGATC58650.032.69193655
CGGATCA59500.032.343356
CTAGATC29850.032.0348745
CGGCCTA1900.031.4128551
AAGCGTC10600.031.133941
CTCGGGG33600.030.5143144
CGTATCA183000.030.41989156
CCGTATC181900.030.41075955
CGAGATC31300.030.3270155
TCTCGGG30900.030.23625643
CATTAAA244600.030.21139761
GGGTCGC42250.029.89713349
TCATTAA243350.029.8345960
CGCCGGA79600.029.71368253
TGTCGTG14750.029.5149271
CAAGATC46850.029.4571915
TCGCGTA3100.029.4458241
TCGCCGG91900.029.16361452
Produced by FastQC (version 0.11.5)