FastQCFastQC Report
Tue 5 Jan 2021
HKVGTAFX2_n02_vk11-13.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHKVGTAFX2_n02_vk11-13.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4210118
Sequences flagged as poor quality0
Sequence length76
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG197330.4687042025900462No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAAGGGG173850.043.81202370
CCCGGTC3400.033.95900354
CGAGATC29300.033.8313185
CCGGATC49850.033.47984755
CGGATCA50450.033.152656
CGGGCTA1600.032.915131
CTAGATC28350.032.6175235
CGTATCA180000.030.98608456
TCTCGGG23550.030.90644843
CGCCGGA67000.030.86187253
CCGTATC179650.030.85021455
CATTAAA231400.030.81672961
CTCGGGG26600.030.51916944
GGGTCGC34550.030.1792749
TCATTAA235500.029.70111560
GCCGGAT73650.029.3586254
CGCCGTA202600.029.18480953
GTATCAT180300.028.79977457
TCGCCGG80050.028.75902452
GATCTCG265850.028.70717841
Produced by FastQC (version 0.11.5)