FastQCFastQC Report
Tue 5 Jan 2021
HKVGTAFX2_n02_vk11-12.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHKVGTAFX2_n02_vk11-12.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5582944
Sequences flagged as poor quality0
Sequence length76
%GC36

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG221100.39602761553760885No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAAGGGG99850.035.5630670
CCGGATC23750.032.85049455
CGGATCA24600.031.85849456
CGCCGGA33200.028.55775653
CCGTATC74550.028.3458855
CGTATCA76050.028.15558256
CGCCGTA82700.027.66719253
GCCGGAT36500.027.41359954
TCGCCGG38100.027.36477952
CGGCAAG9150.027.2495941
CTAGATC16800.027.107645
GTCGCCG123650.026.87933251
GATCTCG114650.026.49281341
TCGCCGT86500.026.41178352
ATCTCGG116200.026.35022442
AGATCTC117100.026.11805540
GCCGTAT92950.025.55719854
CTCGGTG111300.025.52749644
TCGCCCG14300.025.4440551
TCTCGGT109900.025.4399343
Produced by FastQC (version 0.11.5)