FastQCFastQC Report
Tue 5 Jan 2021
HKVGTAFX2_n02_vk11-11.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHKVGTAFX2_n02_vk11-11.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3792391
Sequences flagged as poor quality0
Sequence length76
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG177320.4675678219888192No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGGATC11800.036.7703855
CGGATCA10850.035.79883656
TCGCCGG19200.035.17341252
GGTCGCC36250.034.17203550
GTCGCCG36900.033.2842851
GCCGGAT17300.032.56403754
ATCTCGG25750.031.39220442
ATCGCGG9400.031.27076342
CGCCGGA14100.031.26870353
CGGGTCA11450.031.17240556
GATCTCG25950.031.01500341
GATCGCG9600.030.61888941
TCTCGGG13400.030.03250743
CGCCGTA14200.029.81641853
GCCGGGT34950.029.23439654
CCGTGTC7950.029.0492755
CGCCGGG23750.028.5822653
TGGTCGC25950.028.45251849
TCGCCGT19450.028.42470252
AGATCTC22850.028.0254140
Produced by FastQC (version 0.11.5)