FastQCFastQC Report
Tue 5 Jan 2021
HKVGTAFX2_n02_vk11-10.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHKVGTAFX2_n02_vk11-10.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5759292
Sequences flagged as poor quality0
Sequence length76
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG248780.43196281765189193No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAAGGGG327250.045.98732870
TTCGCGA2750.040.85411
CGAGATC55000.037.1297725
GCGAGAT22400.035.9838644
TCGCGAG5100.035.7755782
CGCGAGA8650.034.8647233
CCGGATC109000.034.5082455
CGCGCAG2450.034.3924831
CGGATCA110100.034.13228656
CTAGATC47950.033.9688455
TGTCGTG23000.032.666631
CGCGAAA6350.032.6209531
CATTAAA405100.032.50248761
TCTCGGG53800.032.32493243
TCATTAA403400.032.08433560
CGTATCA300050.031.94087256
CAAGATC76800.031.8810335
GGATCAT97250.031.84240357
CCGTATC301050.031.70637355
CTCGGGG58650.031.32210744
Produced by FastQC (version 0.11.5)