FastQCFastQC Report
Tue 5 Jan 2021
HKVGTAFX2_n02_undetermined.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHKVGTAFX2_n02_undetermined.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11723269
Sequences flagged as poor quality0
Sequence length76
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG3045932.5981916818593858No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGGATC19800.037.2865855
CGGATCA20700.035.32615756
CCGTATC46450.033.67108555
AAAGGGG82350.033.4041170
TCGCCGG28350.033.07729352
TCTCGGG12700.033.0637243
CGTATCA48200.032.81042556
CGCCGTA51950.032.39708753
GATCTCG72500.032.2891641
CTAGATC13200.032.1064155
CGAGATC15150.031.9041255
TCGCCGT53400.031.38647352
AGATCTC75050.031.19166240
GCCGTAT58500.030.98211354
TCTCGGT68150.030.75637843
CTCGGTG69550.030.59021444
GGATCAT19750.029.40785257
CTCGGGG16300.029.1962744
TAGATCT84250.028.90708239
GTATCAT50750.028.88685657
Produced by FastQC (version 0.11.5)