HKVGTAFX2_n01_vk11-9.fastq.gz FastQC Report

Basic Statistics

Measure	Value
Filename	HKVGTAFX2_n01_vk11-9.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	5127911
Sequences flagged as poor quality	0
Sequence length	76
%GC	37

Per base quality graph

Per base quality graph

Per Sequence quality graph

Per sequence GC content graph

N content graph

Sequence length distribution

Duplication level graph

Sequence	Count	Percentage	Possible Source
CACTCTTTCCCTACACGACGAGATCGGAAGAGCACACGTCTGAACTCCAG	8361	0.16304885166688735	Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
CACTCTTTCCCTACACGAAGATCGGAAGAGCACACGTCTGAACTCCAGTC	6902	0.13459671979486384	Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
CTCTTTCCCTACACGACGAGATCGGAAGAGCACACGTCTGAACTCCAGTC	5880	0.11466657670150672	Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG	5721	0.11156589886212923	No Hit
ACACTCTTTCCCTACACGACGAGATCGGAAGAGCACACGTCTGAACTCCA	5221	0.10181533961880383	Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)

Adapter graph

Kmer graph

Sequence	Count	Obs/Exp Max	Max Obs/Exp Position
ACACTCT	9520	51.867935	1
CGGAGTC	650	37.69184	7
GAAAAAA	36220	36.013836	70
ACGAGTG	615	35.852863	38
GCGTGGA	8945	35.25355	66
GCGTGAA	9085	34.47915	66
CGTGAAA	9180	34.389217	67
GGAAAAA	21325	34.186817	70
GGGTGGA	4730	34.18529	66
GTGGAAA	14090	34.129833	68
GGCGTGA	4780	34.120583	65
CGTGGAA	9375	33.897915	67
GGTCGGC	540	33.702957	61
GCCGTGG	6480	33.48691	56
CCGTGGT	6405	33.059376	57
CGTGGTG	5490	33.02295	58
CGAGTGA	680	32.940445	39
TCGGCGT	8930	32.922	63
TGCGTGG	2885	32.754906	65
CTGCGTG	6460	32.452843	64