FastQCFastQC Report
Tue 5 Jan 2021
HKVGTAFX2_n01_vk11-8.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHKVGTAFX2_n01_vk11-8.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5516403
Sequences flagged as poor quality0
Sequence length76
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACACTCT27350.028.0369431
GGGAAAA56050.023.2914270
TGGCGGG21700.022.74186555
CGGGGGT49900.021.32238649
GCGGGGT15850.020.97785457
GGGGGAA53900.020.64909768
GGGGAAA54600.020.57667269
AGTGCGC3950.020.37949810
GGGGGGA55750.020.02666367
TTGCGGG13450.019.51666855
TGCGGGG16100.019.13037556
CACTCTT63950.019.1085951
ATCGGGG135500.018.8558647
TCGGGGT23800.018.82330148
GAGTGCG4150.018.5539919
AATCGGG141700.018.54952646
TGCCGGG6050.018.51233955
CGGGGTT19750.018.43015549
GGGTGGC22450.018.08441252
CGGGGTG20400.017.84308258
Produced by FastQC (version 0.11.5)