FastQCFastQC Report
Tue 5 Jan 2021
HKVGTAFX2_n01_vk11-7.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHKVGTAFX2_n01_vk11-7.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5013117
Sequences flagged as poor quality0
Sequence length76
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG58410.11651433629017635No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACACTCT59400.048.3992581
GTGAGGT8200.039.26804442
CGAGTGA8400.038.3330939
CGGAGTC8400.037.916057
GAGTCAC8550.037.6602069
ACGGAGT8700.037.417296
ACGAGTG8700.037.0112638
CACGAGT9100.035.3840437
GTCACTC9900.033.5853111
GGGAAAA100950.032.5551970
CGTATGT9950.032.36128258
CACTCTT140900.031.8361171
GGAAAAA79550.031.76566970
GGAGTCA10350.031.1106058
AGTGGCG8150.030.9200539
GGCGGTA7700.030.90889542
GCACTTA10650.030.56288368
GCGGTAG7700.029.99981143
AACGGAG10900.029.5440465
CAAACGG11150.029.5094833
Produced by FastQC (version 0.11.5)