FastQCFastQC Report
Tue 5 Jan 2021
HKVGTAFX2_n01_vk11-6.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHKVGTAFX2_n01_vk11-6.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5548289
Sequences flagged as poor quality0
Sequence length76
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACACTCT35300.032.5365371
GCGTGGA23100.029.09042266
GGGTGGA22350.028.18744766
GCCGTGG18350.024.79522756
GGGCGTG15850.023.8481864
GTGGAAA57700.023.17118868
CACTCTT77850.023.1194061
GTGCGCG3500.022.99961711
GTCGGCG13850.022.743362
GGTCACG4650.022.58026912
TGGGCGT17300.022.25396363
TCGGCGT19850.022.21625363
CCGGGGT20900.022.10489357
TGCCGGG26250.021.866355
TGCCGTG26900.021.8583755
CGTGGAA31400.021.8467767
GCGCGAT3850.021.81781813
GTGGGCG17850.021.76434162
TATGCCG65250.021.50921853
GTGGGGG52000.021.40348862
Produced by FastQC (version 0.11.5)