FastQCFastQC Report
Tue 5 Jan 2021
HKVGTAFX2_n01_vk11-5.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHKVGTAFX2_n01_vk11-5.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5359596
Sequences flagged as poor quality0
Sequence length76
%GC38

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG59640.1112770440160042No Hit
CACTCTTTCCCTACACGACGAGATCGGAAGAGCACACGTCTGAACTCCAG57360.10702299203148895Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACACTCT62950.048.7255631
GGAAAAA146150.034.86780570
CACTCTT159500.033.5437161
AGTGGCG8350.031.85614839
GGGTGGA34350.029.9559266
GGGAAAA158400.029.23255370
CGGAGTC5800.028.9651187
CGAGTGG4750.028.7364562
CCGGGGT31600.027.80025357
ACGGAGT6300.027.7799876
TGCCGGG43150.027.7400655
CGGACAG4850.027.42230248
GAGCTGC9850.027.36003132
ACGAGAG6300.027.2218564
CCGAGTG5050.027.02933361
ACGAGTG6800.026.24988438
TTGCCGG26600.026.18385154
CGGGGTG105150.026.16226658
GCGGTAG9250.025.3512443
TGCGCGA2650.025.09399612
Produced by FastQC (version 0.11.5)