FastQCFastQC Report
Tue 5 Jan 2021
HKVGTAFX2_n01_vk11-4.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHKVGTAFX2_n01_vk11-4.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5452722
Sequences flagged as poor quality0
Sequence length76
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG55370.10154561336521466No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACACTCT63500.045.7658041
GCGTGGA70200.035.8470466
GCCGTGG54350.033.61525756
CGTGGAA75850.033.5921167
CCGTGGT56350.033.2917357
CGTGGTG53050.032.98755358
GGAAAAA224700.032.72536570
GTGGAAA130800.032.3504368
GGGCGTG38900.032.2103164
GGGTGGA52850.031.85382366
GGTCGGC10300.031.60175561
GGTGGGC28050.031.19411361
TGCCGTG76600.030.61311755
TGGGCGT42750.030.2919463
GTCGGCG39300.029.47819762
TCGGCGT47300.029.44987163
GTGGGCG45400.029.37207462
TGGTGGG53400.029.23203860
GCGTGAA37950.029.14317166
CACTCTT161250.028.9228611
Produced by FastQC (version 0.11.5)