FastQCFastQC Report
Tue 5 Jan 2021
HKVGTAFX2_n01_vk11-3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHKVGTAFX2_n01_vk11-3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4156594
Sequences flagged as poor quality0
Sequence length76
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CACTCTTTCCCTACACGACGAGATCGGAAGAGCACACGTCTGAACTCCAG58550.14086052185996514Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG54600.13135754899323823No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACACTCT76150.050.3965721
GGAAAAA124250.038.39381470
GAGTCAC5950.035.293619
CGGAGTC5850.032.905517
GGGAAAA151350.031.58857370
CACTCTT184100.031.4017351
GTGAGGT6250.030.23956342
CGAGTGA6400.030.0776939
GGGTGGA33400.029.7600566
ACGAGTG6400.029.53082338
GTCACTC6650.028.4206411
GGAGTCA7400.027.9050038
ACCGAGC3400.027.79371651
CACGAGT6800.027.79371637
GGTGGGA21000.027.33293767
ACGGAGT7050.027.3075266
CGTATGT7100.027.11228658
GTGGAAA45350.026.85738468
CAAACGG7500.026.6024953
TCGAGGG2900.026.55134256
Produced by FastQC (version 0.11.5)