FastQCFastQC Report
Tue 5 Jan 2021
HKVGTAFX2_n01_vk11-2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHKVGTAFX2_n01_vk11-2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5136164
Sequences flagged as poor quality0
Sequence length76
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACACTCT37050.036.0999071
GCGGGGT40550.026.84327957
TGCCGGG17500.026.59987855
CGGGGTG50150.026.52031758
GGAAAAA94900.026.36948470
GGGAAAA91200.026.36475470
CCGGGGT12950.025.6755657
TGGCGGG58950.025.52999555
GGGTGGA19700.024.8727466
CGGGGTT38600.024.57218749
GTTGGCG23300.024.33465453
TCGGGGT43250.024.03433848
GGTTGGC27050.023.67826552
CACTCTT87650.023.129041
TTGGCGG28450.023.00516754
GGGGAAA88100.022.08821769
TTGCCGG15600.021.7627254
GGGGGAA85150.021.70256668
TGGGGGT33550.021.28136463
GGGGGGA85850.021.28167
Produced by FastQC (version 0.11.5)