FastQCFastQC Report
Tue 5 Jan 2021
HKVGTAFX2_n01_vk11-20.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHKVGTAFX2_n01_vk11-20.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4237930
Sequences flagged as poor quality0
Sequence length76
%GC36

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGGGAAA56050.041.21262770
AAGATCG140050.036.463196
CGGGGGT83350.035.3144451
GAGATCG97650.034.0871546
TCGGGGG249550.034.0808750
ATCGGGG259400.033.94709449
AATCGGG261800.033.80968548
GGGGGAA99850.033.579970
GAATCGG269800.033.02770647
GGAATCG284800.032.5539746
CGGAATC295150.032.5507945
CGTTTCG351150.032.4134340
GTCACGT356400.032.11272436
GTTTCGG353900.032.1022241
ACGTTTC357300.031.99265339
AGTCACG359150.031.97365835
GATCGGA360150.031.9237548
AGATCGG360500.031.9121747
ACGTCTG361450.031.88639822
TCACGTT359450.031.8791937
Produced by FastQC (version 0.11.5)