FastQCFastQC Report
Tue 5 Jan 2021
HKVGTAFX2_n01_vk11-1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHKVGTAFX2_n01_vk11-1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4638636
Sequences flagged as poor quality0
Sequence length76
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG54250.1169524834455646No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACACTCT46050.043.489991
GGCGTGA23100.034.54511365
GGGTGGA30100.033.95315666
GCGTGAA42550.033.56018466
GCGTGGA52050.033.2177366
ACGGAGT5700.033.1597146
GGCGGTA6150.033.00780542
CGGAGTC5900.032.62687
GCCGTGG41550.032.2620256
TCGGCGT44050.032.2584863
CGTGAAA45050.032.16394467
GAGCTGC7000.031.99968732
CGTGGTG37700.031.56467458
ACGAGTG6000.031.4996938
CCGTGGT42550.031.1747857
GGAAAAA140950.031.08873470
GTGGAAA90450.030.95602668
GAAAAAA193800.030.77369370
CGTGGAA57150.030.62087267
CTGCGTG31100.030.27301264
Produced by FastQC (version 0.11.5)